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Schedule

Important Dates

Abstract Submission Deadline:  June 30, 2014

Hotel Booking Deadline:  September 13, 2014

Early Bird Registration Deadline:  September 13, 2014

Cancellation Deadline:  September 19, 2014

 

Monday 13 October 2014

REGISTRATION AND WELCOME RECEPTION

17:00—20:00

Registration and Pick-up of Conference Materials outside the Picadilly Room at the Holiday Inn Executive Center.

18:00—21:00

Welcome Reception in the Picadilly Room at the Holiday Inn Executive Center. Light refreshments will be served.

Tuesday 14 October 2014

7:30-17:00

REGISTRATION
Registration and Pick-up of Conference Materials outside the Windsor I Ballroom at the Holiday Inn Executive Center.

8:00-8:30

WELCOME AND INTRODUCTORY REMARKS
Robert Druce, Conference Chair
Department of Electrical and Computer Engineering, University of Missouri, Columbia, MO USA

ORAL SESSION 1:  MEDICAL APPLICATIONS OF PULSED ELECTRIC FIELDS
Chair:  Stephen Beebe, Old Dominion University, Norfolk, VA USA

8:30-8:50

1) Ex-vivo platelet activation using electric pulses of extended duration
V. Bogdan Neculaes1; Allen L. Garner *2; Antonio Caiafa1 and Andrew Torres1

  1. GE Global Research, Niskayuna, NY 12309, USA
  2. School of Nuclear Engineering, Purdue University, West Lafayette, IN 47907, USA

8:50-9:10

2) Impact of Pulsed Electric Fields in standard platelet components
A.P. Sousa*1; M.T. Pereira2; L.M. Redondo3

  1. Transplant and Blood Portuguese Institute, Lisbon, Portugal
  2. EnergyPulse Systems, Lda., Lisbon, Portugal
  3. Lisbon Engineering Superior Institute, ISEL/GIAAPP, Lisbon, Portugal

9:10-9:30

3) Electrochemotherapy for Head and Neck Cancer in India
Alex A Prasad1; E. Manohara Prasad2; S. Sasi Kumar2; Raji Sundararajan*3

  1. Madras Cancer Center, Chennai, India
  2. OnMed India Pvt Ltd, Chennai, India
  3. Purdue University, USA

9:30-9:50

4) Enhanced Delivery of Plasmid DNA Encoding VEGF by Electrotransfer to Induce Angiogenesis in a Porcine Ischemic Heart Model
Richard Heller*1,2; Anna Bulysheva1; Niculina Burcus1; Cathryn Lundberg1; Robert Strange, Jr.3; Len Muray4; and Barbara Hargrave1,2

  1. Frank Reidy Research Center for Bioelectrics, Old Dominion University, Norfolk, VA, USA
  2. School of Medical Diagnostics and Translational Sciences, College of Health Sciences, Old Dominion University, Norfolk, VA, USA
  3. Naval Medical Center Portsmouth, Portsmouth, VA, USA
  4. Sobran, Inc. Fairfax, VA, USA

9:50-10:10

5) Inactivation of healthcare-associated opportunistic pathogens with shielded sliding plasma discharges on environmental surfaces
Loree C. Heller*1,2; Chelsea M. Edelblute1; Muhammad A. Malik1

  1. Frank Reidy Research Center for Bioelectrics, Old Dominion University, Norfolk, VA, USA
  2. Medical Diagnostic and Translational Sciences, Old Dominion University, Norfolk, VA, USA

10:10-10:30

Break

ORAL SESSION 2:  BASIC RESEARCH
Chair:  Juergen Kolb, INP, Greifswald, Germany

10:30-10:50

1) Imaging and measuring membrane properties on cells by AFM and FRAP gives new insights of electropermeabilization process
Jean-Michel Escoffre1,2; Louise Chopinet1,2,3; Justin Teissié1,2; Cyril Favard1,2 ; Etienne Dague2,3; Marie-Pierre Rols*1,2

  1. CNRS ; IPBS - UMR 5089 ; BP64182, 205 route de Narbonne, F-31077 Toulouse Cedex 4, France
  2. Université de Toulouse ; F-31077 Toulouse Cedex 4, France
  3. CNRS ; LAAS ; 7 avenue du colonel Roche, F-31077 Toulouse Cedex 4, France

10:50-11:10

2) Nanosecond Pulsed Electric Field Exposure Alters the Membrane and Nucleus of Mammalian Cells
Gary Lee Thompson*1; Gleb Tolstykh2; Caleb Roth3; Marjorie Kuipers4; and Bennett Ibey4

  1. Oak Ridge Institute for Science & Education, Fort Sam Houston, TX, USA
  2. General Dynamics Information Technology, Fort Sam Houston, TX, USA
  3. Department of Radiological Sciences, University of Texas Health Science Center at San Antonio, San Antonio, TX, USA
  4. Radio Frequency Radiation Branch, Human Effectiveness Directorate, Air Force Research Laboratory, Fort Sam Houston, TX, USA

11:10-11:30

3) Dependence of the Energy of Electric Pulse, Required to Electroporate the Cells, on Pulse Duration for Pulses from 40 ns to 2 ms
G. Saulis*1,2; A. Bitinaite1,2; R. Saule1; V. Stankevic2; N. Zurauskiene2; S. Balevicius2

  1. Department of Biology, Vytautas Magnus University, 44248 Kaunas, Lithuania
  2. Laboratory of High Power Pulses, Institute of Semiconductor Physics, 01108 Vilnius, Lithuania

11:30-11:50

4) Role of pulse duration on the cellular events induced by PEF
Justin Teissié 

  1. CNRS;IPBS (Institut de Pharmacologie et de Biologie Structurale); 205 route de Narbonne BP64182, F-31077 Toulouse, France
  2. Université de Toulouse; UPS; IPBS; F-31077 Toulouse, France

11:50-12:10

5) Low temperature enhances efficiency of nanosecond pulsed electric fields (nsPEFs) treatment in B16f10 melanoma
Xueming Zhang*; Shengyong Yin; Xinhua Chen; Shusen Zheng

Key Laboratory of Combined Multi-organ Transplantation, Ministry of Public Health, First Affiliated Hospital, School of Medicine, Zhejiang University,310000, Hangzhou, Zhejiang Province, China

12:10-13:30

Lunch

ORAL SESSION 3:  ENVIRONMENTAL APPLICATIONS AND MICROALGAE
Chair:  Shu Xiao, Old Dominion University, Norfolk, VA, USA

13:30-13:50

1) Microalgae Processing at KIT-IHM
W. Frey*1; C. Eing1; M. Goettel1; C. Gusbeth1; M. Sack1; R. Straessner1; C. Posten3; P. Nick2
Karlsruhe Institute of Technology (KIT),

  1. Institute for Pulsed Power and Microwave Technology (IHM), 76344 Eggenstein-Leopoldshafen,
  2. Botanical Institute 1, 76128 Karlsruhe, Germany
  3. Institute of Process Engineering in Life Sciences, Dept. III: Bioprocess Engineering, 76131 Karlsruhe, Germany

13:50-14:10

2) Effect of nsPEF-Treatment on Oil and Astaxanthin Accumulation in Microalgae
C. Gusbeth*1; J. Garrido-Lopez; W. Frey
Karlsruhe Institute of Technology (KIT), Institute for Pulsed Power and Microwave Technology (IHM), 76344 Eggenstein-Leopoldshafen, Germany

14:10-14:30

3) Evaluation of peroxynitrite formation in liquids treated by air plasma
P. Lukes*1; E. Dolezalova1; M. Clupek1; H. Tresp2; S. Reuter2; T. von Woedtke2

  1. Institute of Plasma Physics AS CR, Za Slovankou 3, 18200 Prague, Czech Republic
  2. Leibniz Institute for Plasma Science and Technology (INP Greifswald e.V.), Felix-Hausdorff-Str.2, 17489 Greifswald, Germany

14:30-14:50

4) PowerMod™ Pulsed Electric Field Laboratory Unit Access for Researchers at the Arizona Center for Algae Technology and Innovation’s (AzCATI) Open, Collaborative Algae Testbed
Thomas A. Dempster*1; Michael A. Kempkes2; Ken Schrock2

  1. Arizona State University (ASU), Arizona Center for Algae Technology and Innovation (AzCATI), 7418 E. Innovation Way South, Mesa, Arizona, USA
  2. Diversified Technologies, Inc. (DTI), 35 Wiggins Avenue, Bedford, Massachusetts, USA

14:50-15:10

5) Cell size of microalgae influences the positive impact of pulsed electric field treatment for lipid recovery
Prathap Parameswaran*1; YenJung Lai1; Theresa Rosov2, John McGowen2, Bruce E. Rittmann1

  1. Swette Center for Environmental Biotechnology, The Biodesign Institute at Arizona State University, Tempe, AZ 85287, USA
  2. Arizona Center for Algae Technology and Innovation (AZCATi). Arizona State University Polytechnic campus, Mesa, AZ, USA

15:10-15:40

Break

15:40-16:20

PLENARY #1

High Voltage Atmospheric Cold Plasma Applications in Food
Kevin M. Keener*1; P.J. Cullen2; Paula Bourke3

  1. Purdue University, West Lafayette, Indiana, USA
  2. School of Chemical Engineering, UNSW, Sydney, Australia
  3. School of Food Science and Environmental Health, Dublin Institute of Technology, Cathal Brugha Street, Dublin 1, Ireland

16:30-17:30

Consortium Meeting

17:30-18:30

Poster Setup

19:00-20:30

Business Dinner (by invitation only)

Wednesday 15 October 2014

8:00-17:00

REGISTRATION
Registration and Pick-up of Conference Materials outside the Windsor I Ballroom at the Holiday Inn Executive Center

8:15-8:30

WELCOME AND INTRODUCTORY REMARKS
Robert Druce, Conference Chair
Department of Electrical and Computer Engineering, University of Missouri, Columbia, MO USA

ORAL SESSION 4:  DEVICE DESIGN
Chair:  James VanGordon, bioMerieux, Hazelwood, MO, USA

8:30-8:50

1) Commercializing Pulsed Electric Field Systems and Applications
Michael A. Kempkes*; Ian Roth; Kenneth Schrock

Diversified Technologies, Inc., 35 Wiggins Ave, Bedford MA 01730 USA, www.divtecs.com

8:50-9:10

2) Murine Pulsed Electric Field Generator
Angela Spurgeon*1, Joshua Schorp2, David Lindbloom2, Nicholas Sobol3, Peter Norgard2; Robert Druce2, David Robertson3, Paul Pevsner2

  1. University of Missouri, School of Medicine, Division of Neurological Surgery. Columbia, MO USA
  2. University of Missouri, College of Engineering, Department of Electrical and Computer Engineering, Columbia, MO USA
  3. University of Missouri, Department of Chemistry/MURR, Columbia, MO USA

9:10-9:30

3) Compact Implantable Antenna Incorporating High Dielectric Constant Composite Material
Kevin A. O’Connor*1; Randy D. Curry1; Renee M. Sullivan2

  1. University of Missouri, Center for Physical and Power Electronics, Columbia, MO USA
  2. University of Missouri, Cardiovascular Medicine, Columbia, MO USA

9:30-9:50

4) Generation of Atmospheric-pressure Dry- and Mist -Plasma Jets and Their Effects on HeLa Cells
Douyan Wang*1; Norimitsu Takamura2; Kohei Umeda2; Takao Namihira3; Hidenori Akiyama3

  1. Kumamoto University, Priority Organization for Innovation and Excellence, Kumamoto, Japan
  2. Kumamoto University, Graduate School of Science and Technology, Kumamoto, Japan
  3. Kumamoto University, Institute of Pulsed Power Science, Kumamoto 860-8555, Japan

9:50-10:10

5) Dielectric Spectroscopy of Biological Cells at Physiological Conductivity Using Nanoporous Gold Electrode
Jie Zhuang*1; Lihua Shao2; Anna Steuer1; Juergen F. Kolb1

  1. Leibniz Institute for Plasma Science and Technology, 17489 Greifswald, Germany
  2. Institute of Materials Physics, Hamburg University of Technology, 21073 Hamburg, Germany

10:10-10:30

Break

10:30-12:00

POSTER SESSION 1
Chair:  Xinhua Chen, Zhejiang University, 310000, Hangzhou, China

P-1-1
Biodistribution of Lutetium 177 Nanoconjugates
Joshua Schorp*2, Angela Spurgeon *1, David Lindbloom2, Nicholas Sobol3, Peter Norgard2; Robert Druce2, David Robertson3, Paul Pevsner2

  1. University of Missouri, School of Medicine, Division of Neurological Surgery. Columbia, MO, USA
  2. University of Missouri, College of Engineering, Department of Electrical and Computer Engineering, Columbia, MO, USA
  3. University of Missouri, Department of Chemistry/MURR, Columbia, MO, USA

P-1-2
nsPEFs Inducing Mononuclear Phagocyte Dependent Ablation of Nude Mice Subcutaneous Hepatocellular Carcinoma With Implanted Needle Electrodes
Shengyong Yin*1, Xinhua Chen1, Zhenhua Hu1, Weihua Xu2, Danjing Guo1, Xueming Zhang1, Junxia Yin3, Hangyang Xie1, Lin Zhou1, Shusen Zheng1 

  1. Key Lab of Combined Multi-organ Transplantation, Ministry of Public Health, First Affiliated Hospital, School of Medicine, Zhejiang University, 310003 Hangzhou, Zhejiang Province, China
  2. Institute of Cyber-Systems and Control, Zhejiang University,310027,Hangzhou, China
  3. Health and Family Planning Commission, Tianmen City, Hubei Province, 431700, China

P-1-3

Matrix-Assisted Laser Desorption/Ionization Time-of-Flight Mass Spectrometer for Microorganism Identification
James VanGordon*1; Bradford Clay1; Ron Robinson1; David Pincus1

  1. bioMérieux, Inc, Hazelwood, MO 63042, USA

P-1-4
Molecular Dynamics Simulations for Assessing Plasma Reactive Species Interactions with Lipid Bilayers
Russell S. Brayfield II*1; H. Metin Aktulga2; Ananth Y. Grama3; Allen L. Garner1

  1. School of Nuclear Engineering, Purdue University, West Lafayette, IN 47907 USA
  2. Department of Computer Science and Engineering, Michigan State University, East Lansing, MI 48824 USA
  3. Department of Computer Science, Purdue University, West Lafayette, IN 47907, USA

P-1-5
The Toxicity and Safety Study of Traditional Chinese Herb Pills Sterilized with Nanosecond Pulsed Electric Field
Xinmei Chen*1, Shengyong Yin2, Xudong Ma3, Shifeng Fan3 , Xinhua Chen2

  1. College of Pharmacy Shandong University of Traditional Chinese Medicine, 250355, Jinan, China
  2. Department of Surgery, Zhejiang University, 310000, Hangzhou, China
  3. Xinjiang Nanosecond Pulse Technology Institute, 830000, Urumqi, China, 830000

P-1-6
Temperature Dependence of the Electrical Properties of Biological Cells
Anand Vadlamani*1; Jie Zhuang2; Andrew J. Fairbanks1; Gregory Dunn1; Juergen F. Kolb2; Allen L. Garner1

  1. School of Nuclear Engineering, Purdue University, West Lafayette, IN, 47907, USA
  2. INP Greifswald, 17489 Greifswald, Germany

P-1-7
Cell Suspension Conductivity Variation during Electric Pulses
Andrew J. Fairbanks*, Anand Vadlamani, Tylor Whitmer, Allen L. Garner
School of Nuclear Engineering, Purdue University, West Lafayette, IN, United States

P-1-8
Electric Pulse Induced Changes in Cell Population Dynamics
Tylor Whitmer1*, Anand Vadlamani1, Philip Bardos1, Allen L. Garner1

  1. School of Nuclear Engineering, Purdue University, West Lafayette, IN, United States

P-1-9
Pulsed electric field interactions with primary mixed cortical cultures
Salah Sommakia1; Ashley Baijnath2; Katherine Raun2; Andrew J. Fairbanks2; Kevin Otto1,3; Allen L. Garner*2

  1. Weldon School of Biomedical Engineering, Purdue University, West Lafayette, IN USA
  2. School of Nuclear Engineering, Purdue University, West Lafayette, IN USA
  3. Department of Biological Sciences, Purdue University, West Lafayette, IN USA

P-1-10
Shock Wave Generation for Biological Studies

Shu Xiao*1,2; Dongkoo Kang1, Mohammed Alkhazal2, Michael Cho3

  1. Frank Reidy Research Center for Bioelectrics, Old Dominion University, Norfolk, VA USA
  2. Department of Electrical and Computer Engineering, Old Dominion University, Norfolk, VA USA
  3. Department of Bioengineering, University of Illinois, Chicago, IL USA

P-1-11
Reasons of Cytotoxicity of the Cell Culture Medium Treated with High-Voltage Pulses
L. Zelencova; R. Rodaite-Riseviciene; R. Saule; G. Saulis*

Department of Biology, Vytautas Magnus University, 44248 Kaunas, Lithuania

P-1-12
Bioelectrics Transmission Electron Microscope (TEM) Imaging Facility
David Lindbloom*1, Joshua Schorp2, Angela Spurgeon*3, Paul Pevsner1

  1. University of Missouri, College of Engineering, Department of Electrical and Computer Engineering, Columbia, MO USA
  2. University of Missouri, College of Engineering, Department of Mechanical Engineering, Columbia, MO USA
  3. University of Missouri, School of Medicine, Division of Neurological Surgery. Columbia, MO USA

P-1-13
Differentially Expressed Gene Identification by Microarray Gene Chip Analysis after nsPEF on Hepatocarcinoma
Jian Yu*, Shengyong Yin, Xinhua Chen, Shusen Zheng

Department of Surgery, Zhejiang University, 310000, Hangzhou, China

P-1-14
Biliary duct ligation induces the increase of duodenal glucose absorption in mice
Penghong Song*1, Yehui Du1, Wenfeng Song1, Xiaosun Liu2, Liangjie Hong1, Hui Li1, Haiyang Xie1, Lin Zhou1, Biguang Tuo3, Shusen Zheng1

  1. Key Laboratory of Combined Multi-organ Transplantation, Ministry of Public Health, First Affiliated Hospital, School of Medicine, Zhejiang University, 310003 Hangzhou, China
  2. Department of Surgery, First Affiliated Hospital, School of Medicine, Zhejiang University, 310003 Hangzhou, China
  3. Department of Gastroenterology, Affiliated Hospital of Zunyi Medical College, 563003 Zunyi, China

P-1-15
Mcam silencing with RNA interference using magnetofection or gene electrotransfer has antitumor and antiangiogenic effect
Lara Prosen1; Bostjan Markelc2; Tanja Dolinsek2; Branka Music1; Maja Cemazar2,3; Gregor Sersa*2

  1. Kolektor Group, Nanotesla Institute Ljubljana, Stegne 29, SI-1521 Ljubljana, Slovenia
  2. Department of Experimental Oncology, Institute of Oncology Ljubljana, Zaloska 2, SI-1000 Ljubljana, Slovenia
  3. Department of Natural and Medical Subjects, University of Primorska, Faculty of Health Sciences, Polje 42, SI-6310 Izola, Slovenia

P-1-16
Response of HeLa S3 cells subjected to 120 ns-long and 5 ns-long electrical pulses
Toshiaki Wada*1; Ryuta Andachi1; Sunao Katsuki2, Hidenori Akiyama2

  1. Graduate School of Science and Technology, Kumamoto University, Kumamoto 860-8555, Japan
  2. Institute of Pulsed Power Science (IPPS), Kumamoto University, Kumamoto 860-8555, Japan

P-1-17
Impact of Microalgae Cell Wall and Cell Membrane Disruption by Pulsed Electric Field Processing on the Liberation of Lipids and Phycopigments
Thomas A. Dempster*1

  1. Arizona State University (ASU), Arizona Center for Algae Technology and Innovation (AzCATI), 7418 E. Innovation Way South, Mesa, Arizona, USA

P-1-18
HIFU-based sonodynamic therapy of melanoma cells with Verteporfin
S. Moosavi-Nejad1; R. Naito1; H. Endo1; K. Tachiban1; H. Akiyama1; H. Hosseini*2

  1. Department of Anatomy, Fukuoka University School of Medicine, Fukuoka, 814-0180, Japan
  2. Bioelectrics Department, Institute of Pulsed Power Science, Kumamoto University, Kumamoto 860-8555, Japan

P-1-19
Electroextraction from microalgae by a ms flow process
M. Coustets1,2; E. Luendo3; F. Pillet1,2; C. Mauroy1,2; M. P. Rols1,2; J. Raso3; J. Teissié*1,2

  1. CNRS;IPBS (Institut de Pharmacologie et de Biologie Structurale);205 route de Narbonne BP64182, F-31077 Toulouse, France
  2. Université de Toulouse; UPS; IPBS; F-31077 Toulouse, France
  3. University of Zaragossa, Spain

P-1-20
Nanosecond pulsed electric fields trigger cell cycle arrest and exhibit increased lethality in G2/M Phase
Megan A. Mahlke*1,2; Christopher Navara2; Bennett L. Ibey1

  1. Radio Frequency Bioeffects Branch, Human Effectiveness Directorate, Air Force Research Laboratory, Fort Sam Houston TX, USA
  2. Department of Biology, University of Texas at San Antonio, San Antonio TX, USA

12:00-13:20

Lunch

ORAL SESSION 5:  PULSED ELECTRIC FIELDS FOR CANCER TREATMENT
Chair:  Paul Pevsner, University of Missouri, Columbia, MO, USA

13:20-13:40

1) Nanoelectroablation of tumors leads to the translocation of calreticulin to the cell surface and the initiation of immunogenic apoptosis
Richard Nuccitelli*; Zachary Mallon, Casey Berridge, Mark Kreis, Brian Athos and Pamela Nuccitelli

BioElectroMed Corp. 849 Mitten Rd., Suite 104, Burlingame, CA 94010 USA

13:40-14:00

2) Nanosecond pulsed electric fields abolish orthotopic rat hepatocellular carcinoma and bypass cancer mutations that evade apoptosis induction and immune surveillance
Stephen J. Beebe1, Ru Chen1,2, K. Tyler Harlow1, Nova M. Sain1 and Wei Ren1

  1. Frank Reidy Research Center for Bioelectrics, Old Dominion University, Norfolk VA 23508 USA
  2. Ethicon, 4545 Creek Road, Cincinnati, OH 45242 USA

14:00-14:20

3) Irreversible electroporation and apoptosis of SKOV3 cells can be caused by high-frequency nanosecond pulsed electric fields modulated by microsecond pulse
Yan Mi1; Chenguo Yao1*; Chengxiang Li1 ; Fei Guo2

  1. State Key Laboratory of Power Transmission Equipment & System Security and New Technology, Chongqing University, 400044 Chongqing, China
  2. College of Automation, Chongqing University of Posts and Telecommunications, 400065 Chongqing, China

14:20-14:40

4) Vascular Targeted Effect of Endoglin Silencing Mediated by Electrotransfer in Murine Mammary Adenocarcinoma
Maja Cemazar*1,2; Tanja Dolinsek1; Bostjan Markelc1; Masa Bosnjak1; Tanja Blagus1; Lara Prosen3; Simona Kranjc1; Monika Stimac1; Ursa Lampreht1; Gregor Sersa1

  1. Department of Experimental Oncology, Institute of Oncology Ljubljana, 1000 Ljubljana, Slovenia
  2. Faculty of Health Sciences, University of Primorska, 6310 Izola, Slovenia
  3. Kolektor Group, Nanotesla Institute Ljubljana, 1000 Ljubljana, Slovenia

14:40-15:10

BREAK

ORAL SESSION 5:  Continued

15:10-15:30

5) Calcium electroporation for treatment of cutaneous metastases; a randomized double-blinded phase II study
Julie Gehl*1,2; Hanne Falk1; Louise Wichmann Matthiessen2

Center for Experimental Drug and Gene Electrotransfer (C*EDGE), Department of Oncology, Copenhagen University Hospital Herlev, Denmark

15:30-15:50

6) Electrochemotherapy with bleomycin induces hallmarks of immunogenic cell death in murine colon cancer cells
Lluis M. Mir*1,2,3 ; Christophe Y. Calvet1,2,3; Franck M. André1,2,3

  1. Univ Paris-Sud, Laboratoire de Vectorologie et Thérapeutiques Anticancéreuses, UMR 8203, Villejuif, France-94805
  2. CNRS, Laboratoire de Vectorologie et Thérapeutiques Anticancéreuses, UMR 8203, Villejuif, France-94805
  3. Gustave Roussy, Laboratoire de Vectorologie et Thérapeutiques Anticancéreuses, UMR 8203, Villejuif, France-94805

15:50-16:30

PLENARY 2

Electrochemotherapy of liver metastases – the first clinical experience
G. Sersa*1; I. Edhemovic1; E. Brecelj1; G. Gasljevic1; M. Marolt Music1; V. Gorjup2; B. Mali3; T. Jarm3; B. Kos3; D. Pavliha3; B. Grcar Kuzmanov1; M. Cemazar1; M. Snoj1; D. Miklavcic3; E. M. Gadzijev1

  1. Institute of Oncology Ljubljana, Ljubljana, Slovenia
  2. University Medical Center Ljubljana, Ljubljana, Slovenia
  3. Department of Biomedical Engineering, Faculty of Electrical Engineering, University of Ljubljana, Ljubljana, Slovenia

18:00-18:30

SYMPOSIUM BANQUET RECEPTION

18:30-21:00

SYMPOSIUM BANQUET
Awards banquet in the Picadilly Room at the Holiday Inn Executive Center. Buffet dinner will be served. Guests of participants are welcome to attend for an additional fee.

Thursday 16 October 2014

8:00-17:00

REGISTRATION
Registration and Pick-up of Conference Materials outside the Windsor I Ballroom at the Holiday Inn Executive Center

8:15-8:30

WELCOME AND INTRODUCTORY REMARKS
Robert Druce, Conference Chair

Department of Electrical and Computer Engineering, University of Missouri, Columbia, MO USA

8:30-9:10

PLENARY 3

Influence of pulsed power discharges in water on plants, fishes, foods and Bacteria
Hidenori Akiyama*

Kumamoto University, Institute of Pulsed Power Science, Japan

9:10-9:40

BREAK

ORAL SESSION 6:  BIOLOGICAL EFFECTS OF PULSED ELECTRIC FIELDS
Chair:  Thomas Dempster, Arizona State University, Mesa, AZ, USA

9:40-10:00

1) nsPEF-induced PIP2 depletion, PLC activity and actin cytoskeletal cortex remodeling are responsible for cellular swelling and blebbing
Gleb P. Tolstykh*1, Gary L. Thompson2, and Bennett L. Ibey3

  1. General Dynamics Information Technology, JBSA Fort Sam Houston, Texas, USA
  2. Oak Ridge Institute for Science & Education, JBSA Fort Sam Houston, Texas, USA
  3. Air Force Research Laboratory, 711th Human Performance Wing, Human Effectiveness Directorate, Bioeffects Division, Radio Frequency Bioeffects Branch, JBSA Fort Sam Houston, Texas, USA

10:00-10:20

2) Activation of Find-Me and Eat-Me Signaling Pathways After nspef Treatment of Tumor Cells
Chao Cen*, Shengyong Yin, Xinhua Chen, Shusen Zheng

Department of Surgery, Zhejiang University, 310000, Hangzhou, China

10:20-10:40

3) Difference of caspase activity for frequency components of pulsed electric field on budding yeast
Yasushi Minamitani*; Riku Kageyama; Yuta Kobayashi; Kenji Tamura

Graduate School of Science and Engineering, Yamagata University, Yonezawa, Yamagata, 992-8510, Japan

10:40-11:00

4) Calcium electroporation of spheroids induce cell death in cancer cell lines, not a normal cell line
Stine Krog Frandsen*1#, Laure Gibot2#, Moinecha Madi2, Julie Gehl1, Marie-Pierre Rols2

  1. Department of Oncology, Copenhagen university Hospital Herlev, Denmark
  2. Institute of Pharmacology and Structural Biology, CNRS, Toulouse, France

# These authors have contributed equally to this work

11:00-11:20

5) Calcium-dependent cellular responses to nanosecond pulsed electric fields
Ken-ichi Yano* and Keiko Morotomi-Yano

Department of Bioelectrics, Institute of Pulsed Power Science, Kumamoto University,
Kumamoto 860-8555, Japan

11:20-11:40

6) Calcium flux and impedance spectra of cells treated by induced nanosecond pulsed electric fields
A.J.M. Pemen*1, Yin Pan1; J.W.M. van Bree2, A.M.C.B. Koonen–Reemst2

  1. Eindhoven University of Technology, Department of Electrical Engineering, PO Box 513, 5600 MB Eindhoven, The Netherlands, a.j.m.pemen@tue.nl
  2. Vabrema B.V., Eindhoven, The Netherlands

11:40-13:00

LUNCH

13:00-13:40

PLENARY 4

Ion and small molecule transport through lipid electropores
P. Thomas Vernier*1; James C. Weaver2

  1. Frank Reidy Research Center for Bioelectrics, Old Dominion University, Norfolk, VA, 23508 USA
  2. Harvard-MIT Division of Health Sciences and Technology, Massachusetts Institute of Technology, Cambridge, MA, 02139 USA

ORAL SESSION 7:  MODELING AND SIMULATION
Chair:  Jie Zhuang, INP, Greifswald, Germany

13:40-14:00

1) Calculations of Plasma Membrane Temperature Gradients Due to Electric Fields
Allen L. Garner*1; Maxim Deminsky2; V. Bogdan Neculaes3; Boris Potapkin2

  1. School of Nuclear Engineering, Purdue University, West Lafayette, IN 47907, USA
  2. Kintech LTD and NRC “Kurchatov Institute,” Moscow, Russia
  3. GE Global Research, Niskayuna, NY 12309, USA

14:00-14:20

2) Electric Pulse Induced Membrane Voltage with Capacitive Coupling
Vance Robinson1; Allen L. Garner*2; V. Bogdan Neculaes1

  1. GE Global Research, Niskayuna, NY 12309, USA
  2. School of Nuclear Engineering, Purdue University, West Lafayette, IN 47907, USA

14:20-14:40

3) Numerical Modeling of Irreversible Electroporation for Cancer Treatment
E.L. Latouche, R.V. Davalos*

Biomedical Engineering, Virginia Tech, Blacksburg, VA, USA

14:40-15:00

BREAK

ORAL SESSION 8:  PULSED ELECTRIC FIELD STIMULATION
Chair:  Rafael Davalos, Virginia Tech University, Blacksburg, VA, USA

15:00-15:20

1) Electrical Stimulation by Subnanosecond Pulses
Shu Xiao*1,2; Iurii Semenov1, Dongkoo Kang1, Andrei Pakhomov1, Karl H. Schoenbach1

  1. Frank Reidy Research Center for Bioelectrics, Old Dominion University, Norfolk, VA USA
  2. Department of Electrical and Computer Engineering, Old Dominion University, Norfolk, VA USA

15:20-15:40

2) Nanosecond Pulsed Electric Fields Alter Gap Junction Mediated Intercellular Communication
Anna Steuer*; Jie Zhuang; Juergen Kolb

Leibniz Institute for Plasma Science and Technology (INP Greifswald e.V.), Felix-Hausdorff-Str. 2, 17489 Greifswald, Germany

15:40-16:00

3) Mechanisms of shock wave-induced traumatic brain injury: Experimental and analytical evaluations
H. Hosseini*1; H. Akiyama1; V. Menezes2; S. Moosavi-Nejad3 

  1. Bioelectrics Department, Institute of Pulsed Power Science, Kumamoto University, Kumamoto 860-8555, Japan
  2. Dept. Aerospace Eng., Indian Institute of Technology Bombay, Powai, Mumbai 400076, India
  3. Department of Anatomy, Fukuoka University School of Medicine, Fukuoka, 814-0180, Japan

16:00-16:20

4) Behavior of Proteins Under Non-thermal Large Electric Fields
Sunao Katsuki*1; Daisuke Akiyama2; Masaya Morodomi2; Hidenori Akiyama1

  1. Institute of Pulsed Power Science (IPPS), Kumamoto University, Kumamoto 860-8555, Japan
  2. Graduate School of Science & Technology, Kumamoto University, Kumamoto 860-8555, Japan